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mRNA expression analysis by real-time PCR Total RNA was isolated from the dissected tissues using the RNeasy Mini kit (Qiagen, CA, USA), followed by reverse transcription using the High Capacity RNA-to-cDNA kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturers instructions

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Two metabolites, coumarin and L-leucine, exhibited higher relative abundance in ASD samples compared to controls, with fold changes of 6.87 (logFC = 2.78) and 3.26 (logFC = 1.71), respectively

Recent advances in single-cell transcriptomics and spatial omics technologies have provided deeper insights into CSC-specific gene expression patterns, leading to the discovery of more refined CSC markers

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By reducing these organic compounds, BAC minimizes the potential for bacteria to grow in the water supply, helping to maintain water quality as it travels through pipelines and reaches consumers