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100 ng/l BSA (ThermoFisher, Cat# AM2614)) supplemented with either 40 mM KCl (fragments 1093-1172, 1158-1237, 1230-1315, 1308-1394, 1391-1447) or 100 mM KCl otherwise

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The 'LR3' designation refers to a 13-amino-acid N-terminal extension and a glutamic acid substitution at position 3, which together reduce binding affinity for IGF-binding proteins (IGFBPs) by approximately 100-fold