383 SIRT5-catalyzed demalonylation and inactivation of SDHA block the TCA cycle and induce succinate accumulation, promoting tumorigenesis and drug resistance
The homogenates were centrifuged (Eppendorf 5810R, Hamburg, Germany) at 1000 g at 4 C for 10 min
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Both require identical refrigeration: 36-46F before use
Step 3: Consistency is key
At 3-month follow-up, the patient continued to use a wheelchair but had continued slow improvement in motor function, and he reported no significant residual back pain