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Three distinct clades were identified that contain: plant TyrAs together with those from algae, spirochaetes, - and -proteobacteria ( clade I , shaded blue in Figure 2, Figure S1), TyrA orthologs from some archaea, fungi, -proteobacteria, and chloroflexi ( clade II , shaded green), and TyrA orthologs from various microbes, which formed the outgroup and contains previously characterized microbial TyrA orthologs from Synechocystis sp

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