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The model also highlighted significant changes in intracellular metabolite concentrations and pH, reflecting typical cellular adaptations to hypoxia

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Cell supernatants and lysates were plated on 7H11 agar medium (Hi Media, Santa Maria, CA, USA) enriched with ADC (GEMINI, USA) and incubated at 37C for three weeks to evaluate mycobacterial growth or survival under treatment conditions by counting the colony-forming units (CFUs)