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Urine , the output of the collecting ducts, is a solution of released electrolytes, nitrogen compounds, and other metabolic waste

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Alkylating agents such as NEM or iodoacetamide can be added to block free thiols and lock the GSH/GSSG ratio at the time of collection

This makes them vital for transmitting signals from insulin and IGF receptors to downstream effectors, which control cellular activities such as glucose uptake and protein synthesis [90, 113]

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