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In each of the tubes, 125 L of trichloroacetic acid (TCA 20%) and 250 L of thiobarbituric acid (TBA 0.67%) were added

UK guidance recommends monitoring clinical response rather than routinely rechecking serum B12 levels once treatment is established

doi: 10.1016/S0264-410X(03)00159-2 53 MoAXColleyDG

The initial enzyme reaction rate ( V 0 ) was estimated from the absorbance ratio of the mixture before and after reaction of 10 min, and a nearly linear plot of V 0 versus the concentration of GST was observed (Fig