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Testing The Methylation Genes The first step in personalized MTHFR management is to understand your specific genetic variations

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Once colonies were found, competent cells were prepared and transformed with a URA resistance cassette with homology arms to endogenous CHC1 gene (See Supplementary Table 5 for primer S2/S3 sequence) using the established PCR cassette protocol (pFA6a-KlURA3 Vector) 65

The S375A mutation strongly impairs DAMGO-driven MOR endocytosis (Schulz et al., 2004)