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ESI-MS study of the mechanism of glycyl-l-histidyl-l-lysine-Cu(II) complex transport through model membrane of stratum corneum

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The peptide's molecular weight (340 Da in its copper-bound form) places it well below the glomerular filtration threshold of approximately 60,000 Da, meaning it passes freely through the kidneys and appears in urine within 24 hours of administration

The dose-response relationship with sleep disruption appears roughly linear: higher doses correlate with more pronounced effects