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Samples must be collected within two to four hours post-administration to capture measurable concentrations, and immediate processing (centrifugation, plasma separation, freezing at 80C) is essential to prevent ex vivo degradation
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Keep ambient-temperature handling time as short as practical to support compound integrity
CONCLUSION: The apparent volume of distribution suggests a predominantly extracellular partitioning of the antidote, even in the presence of cyanide, an important factor in terms of its antidotal effect
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