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The harvested cells were resuspended in buffer A (20 mM Tris-HCl, pH 8.0, 300 mM NaCl and 1 mM DTT) for GST-tagged proteins or in buffer B (20 mM Tris-HCl, pH 8.0, 300 mM NaCl, 5% glycerol and 10 mM imidazole) for His-tagged ShHTL7, and they were lysed by sonication
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It has been proven through the utilization of cell-based and transgenic animal models that high-risk variants are responsible for cellular damage and mortality, whereas the reference APOL1 G0 is relatively non-toxic (107, 216)
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