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Drug Delivery 30:2241661 Jaiswal AK (2004) Nrf2 signaling in coordinated activation of antioxidant gene expression

Briefly, RAW 264.7 cells were washed three times with phosphate buffer saline (PBS, Corning) and then incubated in ice-cold hypotonic lysing buffer (50 mM TrisHCl, 10 mM magnesium sulfate at pH 7.5) for 4 h at 4 C

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Withdrawal symptoms can be managed through awareness of the withdrawal reactions, individualized taper strategies according to withdrawal severity, the addition of alternative strategies such as reassurance, and referral to benzodiazepine withdrawal support groups