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The NHS recommends a balanced diet and regular physical activity as the best approach for weight loss

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Thus, sections were deparaffinized, rehydrated and subjected to antigen retrieval with citrate buffer, pH 6, containing 0.05% (vol/vol) Tween-20 (Sigma-Aldrich, Darmstadt, Germany), cooled in distilled water and permeabilized in 0.4% (vol/vol) Triton-X-100 in phosphate-buffered saline (PBS), pH 7.4, on ice for 30 min

Reduced dietary intake is the primary driver

Oxidative medicine and cellular longevity, 2017, 1716701