TUDCA is believed to increase energy burned at the cellular level, potentially affecting how the body regulates insulin
Permeabilization was then performed using PBS containing 0.5% Triton X-100 (108,603, Merck, Darmstadt, Germany) for 1 h at room temperature, followed by washing twice for 5 min in PBS/BSA
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PIT SWEEPER PACKAGE (OPTIONAL) A PVC pit sweeper is installed in the pit to keep particles suspended and swept toward pump suction for removal
After coincubating freshly isolated and activated mouse T cells with particles encapsulated with EGFP or luciferase mRNA for 24 h, the CD5/ LNP group exhibited significantly higher EGFP or luciferase expression rates than did the unmodified LNP group (Fig