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After adding samples and biotinylated antibodies, Avidin-peroxidase conjugates were added

Micrographs were processed using the Patch Motion Correction and Patch CTF Estimation jobs, then manually curated to remove micrographs with CTF fit resolution cutoffs greater than 5

Mitochondria affect ferroptosis at the level of the cytosolic iron pool regulation by fine-tuning the iron influx from the cytosol into mitochondria, at the level of electron transport chain by adjusting mitROS generation and oxidative phosphorylation via AMPK activation in the energy-deficient state, and at the level of tricarboxylic acid (TCA) cycle affecting the content of NADPH and GSH [162, 166]

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