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While in the case of of semaglutide mutant 524 (supplementary file 4452.pdb ) here, one clear cut interfacial salt bridge was formed at 3.122 A (Table 4) between Arg127 in semaglutide [24, 35]) and Glu40 of GLP-1R ECD, and another one also categorized here as an interfacial salt bridge at 4.023 A, as the inter-actomic distance (4.023 A, Table 4) equals almost the cut-off distance (4.0 A) for salt bridge analysis as used in [34]

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