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Using the Broad Institute's Connectivity Map (CMap), which compares gene expression signatures across thousands of compounds, Pickart et al

The combination produces a synergistic research signal larger than either compound alone at equivalent total doses

The reaction was started with addition of an equal volume of DTNB (6 mM) and NADPH (100 M) and the increase in A 412 during the first 3 min was recorded

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