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Neuroprotection Against Parkinsons Models MPTP selectively destroys nigrostriatal dopaminergic neurons, producing tremor, rigidity, akinesia, and catalepsy in rodents

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Marina RJ, Brannan KW, Dong KD, Yee BA, Yeo GW

mRNA expression analysis by real-time PCR Total RNA was isolated from the dissected tissues using the RNeasy Mini kit (Qiagen, CA, USA), followed by reverse transcription using the High Capacity RNA-to-cDNA kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturers instructions

Food stays in the stomach longer while the brain receives amplified satiety signals, producing the sensation of fullness that can tip into nausea when eating patterns aren't adjusted