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Using 2mL of water simply dilutes the solution more, making it easier to measure smaller doses with greater accuracy because you're drawing a larger volume

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Impaired individuals exhibit hypomethylation of CpG sites in the CCL11 promoter region, which are then hypermethylated upon supplementation with B12 In impaired individuals, three CpG sites exhibited hypomethylation when compared to the non-impaired group