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After 24 h transfection, cells were washed twice and incubated with blocking buffer (F12 supplemented with 33 mM HEPES, and 0.1% (w/v) BSA, pH 7.4) for 2 h at 37 C

You may inject in the same body area each week, but it is important to use a different spot each time

Available from Accessed 18 Jan 2022 Clarke PM, Fitzgerald EM (2010) Expiry of patent protection on statins: effects on pharmaceutical expenditure in Australia

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Current clinical guidance strongly discourages their use