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Step 3: Pick a time and stick to it

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Tian et al., 1997

10.1210/jcem.82.4.3901 52 HolzenbergerM.DupontJ.DucosB.LeneuveP.GlonA.EvenP

2.80g of Fmoc-Pro-OH, 1.13g of HOBt and 1.2mL of DIC are weighed, DMF20mL is added for dissolution, and activation is carried out for 10min in ice bath