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Cells were thawed and resuspended in purification buffer (250 mM KCl, 2 mM MgCl 2 , 40 mM HEPES-KOH pH 7.9, 5 mM -mercaptoethanol, 10% (v/v) glycerol) supplemented with protease inhibitors and DNase I before lysis by successive cycles of freeze-thaw

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