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Description

786-O cells were cultured in RPMI +pyruvate +FBS [RPMI containing 1 mM pyruvate and 25 mM glucose (ThermoFisher, A1049101) supplemented with 10% non-dialyzed FBS and 1% penicillin/streptomycin] at 37C in 5% CO 2

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The dilution of the ELF was calculated by measuring urea in both the BAL fluid (BALF) and plasma as previously reported [16]

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Mammalian G proteins and their cell type specific functions

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