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Methods Cells The 293 T cells (National Collection of Authenticated Cell Cultures, Shanghai, China) were cultured in Dulbeccos modified Eagles medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 100 U/ml penicillin-streptomycin (Gibco) at 37 C with 5% CO 2

Scenario 2: Using 2mL of BAC Water This creates a more diluted solution, which can be fantastic for protocols requiring greater precision with smaller doses

USA 98 , 94259430 (2001)

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Recently, it has been shown that overexpression of ACSL4 reverses the myocardial protective effect of baicalin on ischemiareperfusion and amplifies the degree of myocardial lipid peroxidation [79]