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Cells were imaged in a Nikon spinning disk field scanning confocal microscope using a 100X oil objective under TIRF illumination with the following microscope settings using the MetaMorph software: 488 nm excitation laser, exposure time of 150 msec, 10% laser power at a rate of 1 frame per second for 150 frames

Fatty-acid side chains and other structural modifications can complicate both synthesis and downstream purification

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These indicators also form the foundation for clinical metabolic-syndrome screening a reminder of why shoppers scrutinize sugar, fiber and protein on labels today

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