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Following centrifugation at 10.000 g for 15 min at 4 C, the resulting supernatant was collected for the determination of IL-1, TGF-, MMP-2, MMP-9 and TIMP-1

A glucose target of 7 may do better if their glucose is allowed to drift higher (up to ~250 mg/dL)(13.9 mM). When in doubt, dose insulin conservatively

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