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Oxidative stress imaging: Visualizing oxidative cellular stress induced by nanoparticles in the subcytotoxic range using fluorescence lifetime imaging (small 23/2018)
Bohnen et al
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Understanding which peptides cross the BBB, and by which route, is essential for anyone evaluating peptide dosing protocols for neurological applications
Cell culture and bioluminescence analysis T cells, isolated from mice, were activated by CD3/CD28 Streptamer kit (#6-8920, iba, Goettingen, Germany) for 30 min and cultured in RPMI 1640 medium (#8123340, Gibco) containing 10% FBS (#A5669701,Gibco), 1% penicillin/streptomycin, 50 M -mercaptoethanol (Beyotime, Shanghai, China), 1 mM sodium pyruvate (Beyotime) and 100 U/mL interleukin-2 (#CK24, Novoprotein, Suzhou, Jiangsu, China)