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Inject the water SLOWLY along the vial wall

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A 100 mL aliquot of homogenate was then removed and added to a reaction mixture containing 200 mL 8.1% ( w / v ) lauryl sulfate, 1.5 mL 20% ( v / v ) acetic acid (pH 3.5), 1.5 mL 0.8% ( w / v ) thiobarbituric acid and 700 mL distilled water

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