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Renal function categories were based on the eGFR as per Chronic Kidney Disease Epidemiology Collaboration

After overnight incubation, the medium was changed to 90% of Williams E Medium and supplemented with 12.5% HepaRG preinduction and toxicity medium supplement (Lonza, catalog: MHPIT) and 25 U/mL penicillin-25 g/mL streptomycin, followed by 72 h incubation

Sudden and severe flare-ups and symptoms such as fever, swollen lymph nodes, and skin tenderness characterize it

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Importantly, this study also demonstrates limited impacts of metformin treatment on the metabolic profile of non-cancer rectal tissue, supporting its potential utility as a radiosensitising agent in the management of rectal cancer