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Its molecular structure was optimized through genetic engineering technology: an additional 13 amino acids were added to the N-terminus of natural IGF-1 (70 amino acids, molecular weight 7.4 kDa) to form a long-chain structure of 83 amino acids (molecular weight 9.11 kDa), and the third glutamic acid (Glu) was replaced with arginine (Arg), hence the name "R3"

2) revealed that expression of these genes were not different between WT and KO livers in any diet groups