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Peptides were then eluted from the StageTip in eight fractions using 20 mM ammonium formate buffers with increasing (v/v) concentrations of acetonitrile (5%, 7.5%, 10%, 12.5%, 15%, 20%, 25% and 45%)

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Once the expression of functional endogenous HLA-DRB alleles had been eliminated, we next embarked to create a stable cell line expressing an exogenous BCR capable of binding all human IgG subclasses

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