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Western blotting Total protein was extracted from paraformaldehyde-fixed, paraffin-embedded pancreatic tissues using the FFPE Total Protein Extraction Kit (Sangon Biotech, Shanghai, China) or from cultured PSCs using RIPA Lysis Buffer (Beyotime)

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Similarly, SINEUPs are novel interventions designed to bind with the non-coding region of m-RNAs, hence they prevent these molecules from degradation, increasing their half-life and enhancing the production of proteins that work on disease pathways