MATERIALS AND METHODS Animals Male Albino Wistar (200 g) rats, were used in all of the experiments approved by the Local Ethic Committee (at least 10 rats per each experimental group per each period), assessed by observers naive about the given treatment
Mayo Foundation for Medical Education and Research (MFMER)
The procedure is carefully controlled for temperature and timing, with incubations typically ranging from 1 to 2 h at room temperature to overnight settings at 4C, depending on the target analyte
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for instance, the exact cellular localization of Scly, whether Scly directly transfers Se to selenophosphate synthetase 2 (SEPH2) or there are other proteins involved, and the exact role of minor intermediates such as selenohomocysteine (trans-selenation pathway) or Se bound to glutathione (selenite reduction pathway) in Se turnover (Seale et al., 2018a
Int J Radiat Oncol Biol Phys 61(3):656-664, 2005