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Additionally, the width of the distribution ( Span ) was calculated based on the distribution of diameters of the droplets at which 90%, 50% or 10% of the sample was smaller than the size measured (Dx (10), Dx (50), and Dx (90), respectively) according to our previous study 18

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Liquid chromatographymass spectrometry peak extraction and integration were analyzed with commercially available software Sieve 2.0 (Thermo Scientific)

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These are not protocol prescriptions

Pi-Sunyer, X