After microscopic examination, oocytes at metaphase II stages (MII, with the polar body extrusion) were collected and cultured in 500 l of fertilization droplets as SOF medium supplemented with 5% fetal bovine serum, 2 mM sodium pyruvate, 10 g/ml heparin sodium and 1 mM caffeine in a CO 2 incubator (38.5C, 90%N 2 , 5%CO 2 and 5%O 2 ) (32)
Ein kompetenter, freundlicher Arzt, der sich Zeit nimmt, zuhrt und ein Vertrauensverhltnis aufbaut
Anderson Cancer Center Experience
We do not administer injections without a proper assessment
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Clin Nutr ESPEN (2022) 51:8391