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TSA was carried out using a BioRad CFX Connect qPCR instrument with a melt curve setting of 25 95 C, in increments of 0.5 C/10 seconds

CO suppresses NLRP3 inflammasome activation, reduces pyroptosis, scavenges ROS, and enhances DNA repair, while Mn alleviates hypoxia via HO-to-O conversion and downregulates fibrotic markers (e.g., TGF-1, -SMA)

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However, further research is needed to better understand the potential consequences of stopping glutathione supplementation, and individual responses may vary