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All cells were cultured in an endothelial cell-specific medium for human umbilical vein endothelial cells, which was supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (PCM-H-040, ZQXZbio, China)

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This multidisciplinary approach, which addresses both the biological and physical dimensions of tissue healing, ensures that each aspect of the regenerative processfrom cellular signaling and extracellular matrix remodeling to the mechanical environmentis holistically managed, providing an advanced strategy for medical treatment and rehabilitation

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FOXO4-DRI selectively induces apoptosis in senescent cells by disrupting the FOXO4-p53 interaction that keeps these damaged zombie cells alive, without harming healthy cells