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Pierce, WI, USA) was performed during the second wash step, followed by a final wash with 0.1 M PBS

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Similar content being viewed by others 1 Introduction Vitamin B12 is essential for DNA synthesis, methylation, and folate metabolism, especially to convert folate to its active form, synthesize purines, convert homocysteine into methionine, and produce S-adenosylmethionine, which is involved in over 100 methylation reactions