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RNA isolation, cDNA synthesis, and quantitative RT-PCR H9c2, NMCMs, and hiPSC-CMs were collected into QIAzol Lysis Reagent or Trizol Reagent (Invitrogen) and total RNA was extracted using Direct-zol RNA Miniprep and Microprep (Zymo Research, CA, USA), respectively

Furthermore, hyperhomocysteinemia is involved in the etiology of migraine (15)

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2024;391(17):1573 Hongyi Z, Lenian Z, Qiuke W, Qianying C, Fan Y, Hanqiang J, Yiwei C, Yanyan S, Changqing Z

Researchers couldnt make the IGF-diabetes association in people over age 65 Better Heart Health In 15-years prospective study of 231 cases and 374 matched controls without any sign of ischemic heart disease (IHD) at the baseline of the study, those with baseline IGF-I levels in the lowest quartile had a 2-fold increased risk of IHD (when the major IGF-binding protein, IGFBP-3, was controlled)